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KMID : 0364820140500010084
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Korean Journal of Microbiology
2014 Volume.50 No. 1 p.84 ~ p.86
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Fermentation of a Potential Biocontrol Agent, Bacillus amyloliquefaciens SKU-78 Strain
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Kim Shin-Duk
Cho Hong-Bum
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Abstract
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Mass production of biocontrol agent is an essential step for its commercial use. Media composition and culture conditions for production of Bacillus amyloliquefaciens SKU-78, a potential biocontrol agent against bacterial wilts, were optimized by a flask culture. Low cost media combining nitrogen and carbon sources were tested. Maximum cell growth (>2 × 109 CFU/ml) was obtained in a medium of 5% soy flour combined with 3% corn starch after 24 h cultivation. The optimum initial pH, temperature and shaking speed was 5.5, 30℃ and 150?250 rpm, respectively. Fermentation of SKU-78 was scaled up in 30 L fermenter and the profiles of cell density, pH, dissolved oxygen and spore formation were recorded. After 8 h lag phase, exponential growth occurred and reached at maximum viable cell number (1.2 × 1011 CFU/ml) after 20 h. The SKU-78 strain grown in a low cost medium exhibited the high suppression of bacterial wilts. The results indicate that SKU-78 strain can be produced in a low cost medium and provide a basis for scaling up to industrial level.
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KEYWORD
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Bacillus amyloliquefaciens, bacterial wilt, biocontrol agent, media optimization
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